Port Moresby's Santos National Football Stadium will host the Australian and PNG Prime Minister's XIII teams on October 3.
The Australian Rugby League Commission and Prime Minister Anthony Albanese announced both Australian squads.
Ben Te'o will coach the men, while Jillaroos coach Jess Skinner leads the women.
The men's squad brings together emerging talent and established players.
Commission chairman Peter V'landys congratulated those selected, saying they would represent Australia as ambassadors.
"Not only do they have the opportunity to represent their country, they will be ambassadors for Australia and for Rugby League. They will change lives for the better."
Skinner said, "With the women's international game going from strength to strength, the players can't wait for the game."
Men's selections are William Kennedy, Jojo Fifita, Jaxon Purdue, Nick Meaney, Braidon Burns, Ezra Mam, Lachlan Galvin, Toby Couchman, Billy Walters and Josh Curran.
Also selected are Ryan Couchman, David Fifita (captain), Kurt Mann, Tallyn Da Silva, Owen Pattie, Nathan Brown, Tom Chester, Jock Madden, Josh Kerr and Hamish Stewart.
Women selected are Ahlivia Ingram, Andie Robinson, Bree Chester, China Polata, Chloe Jackson, Georgia Hannaway, Jacinta Carter, Jada Taylor, Jasmin Morrissey and Kasey Reh.
The squad also includes Khyliah Gray, Lucy Spain, Montana Clifford, Rory Owen, Ruby-Jean Kennard-Ellis, Tahlia O'Brien, Taneka Todhunter, Tayla Preston, Tess McWilliams and Tori Shipton.