Julia Robinson will miss the rest of the World Cup after dislocating her wrist against England, with scans later revealing ligament damage but no fracture.
The injury has brought Canberra Raiders back Sheridan Gallagher into the Jillaroos' extended squad for Sunday's match against Wales.
Australia arrive in Sydney seeking another shutout after beating Samoa 40-0 and England 78-0.
Wales have lost 70-6 to England and 76-0 to Samoa, prompting bookmakers to expect another difficult match.
Coach Jess Skinner has rotated the squad, resting some players and giving others a chance before the semi-finals.
Jesse Southwell will start at five-eighth in place of Jocelyn Kelleher.
"It's something you dream of as a kid," Southwell said at training on Wednesday.
"This will be my first time in a World Cup, it's so amazing and definitely a pinch me moment," she added.
Forward Keilee Joseph said she was ready to take any opportunity and make her family proud.
"(I'm) doing it for my family and doing it in front of my family to make them proud," Joseph said.
"I started playing when I was 11, and that was on the back of my mum pushing me into playing it.
"Wherever they need me, I'll be there."